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½ÄÇ°È¿¼ÒÇй׽ÇÇè ±â¸»½ÃÇè (15³â 2Çбâ) Çаú(ºÎ): Çйø: ¾ÏÈ£: À̸§: ( )¿¡ µé¾î°¥ Àû´çÇÑ ´Ü¾î¸¦ ¿µ¾î´Ü¾î·Î ÀûÀ¸½Ã¿À(°¢ 2Á¡). 1. ( ) is the biocatalyst which speed up the rate of biochemical reaction in the living cells. 2. In the early ancient Egypt and Greece, people used ( ) on the surface of grape to make wine. The ( )'s enzyme change the glucose to alcohol. 3. Emil Fisher had performed some classical studies on carbohydrate metabolizing enzymes in which he demonstrated the specificity shown by an enzyme for its substrate. On the basis of this experiments, Fisher proposed the ( ) hypothesis. This explains the enzyme specificity. Each enzyme works for certain specific reaction. 4. In 1965, three dimensional structure of lysozyme was deduced by ( ). This makes possible to postulate the enzyme mechanism of action in precise structural terms. ´ÙÀ½À» ¼³¸íÇϽÿÀ. ´Ü äÁ¡½Ã ÃÖ°íÁ¡¼ö¿¡ ´ëÇÏ¿© »ó´ëÀûÀ¸·Î äÁ¡À» ÇÒ ¿¹Á¤ÀÌ´Ï ÀÚ¼¼ÇÏ°Ô Á¤È®ÇÏ°Ô ´äÀ» ÇϽÿÀ. 5. »ýü¿¡¼ À¯¹ßÇÒ ¼ö ÀÖ´Â ´ë»çÀÌ»ó¿¡ ´ëÇÏ¿© ´ÙÀ½À» ±â¼úÇϽÿÀ. (10Á¡) 1) ´ë»çÀÌ»óÀÇ ¿øÀÎÀº ¹«¾ùÀΰ¡? 2) ±× Á¾·ùÀÇ ¿¹¸¦ 1°¡Áö ÀÌ»ó ¿°ÅÇϽÿÀ. 3) Ä¡·á ¹æ¹ýÀº? 6. ³ëÈ¿¡ ´ëÇÑ ¿øÀÎ °¡¼³À» ¼³¸íÇØ º¸rh ÀÌÀÇ ±Øº¹ ¹æ¹ýÀ» ±â¼úÇϽÿÀ. (10Á¡) 7. À½½Ä¹°ÀÇ ¼ÒÈ¿Í Èí¼ö¿¡ ´ëÇÏ¿© ´ÙÀ½À» ¼³¸íÇϽÿÀ. 1) ³ª³ëÇÐÀû °üÁ¡¿¡¼, 2) È¿¼ÒÇÐÀû °üÁ¡¿¡¼ ¼³¸íÇϽÿÀ. (10Á¡) 8. »ê¾÷¿ë È¿¼Ò(½ÄÇ°, ÀÇ¾à ¿ëµµ) °³¹ßÀ» À§ÇÏ¿© °¡Àå ÇÊ¿äÇÑ ±â¼ú°ú ÀÌÀÇ ´ëó Àü·«Àº? (10Á¡) 9. È¿¼Ò»ý»ê±ÕÀÇ ºÐ¸®¿¡¼ Ž»ö(screening and selection)ÀÌ Áß¿äÇÑ ÀÌÀ¯´Â ¹«¾ùÀΰ¡? (10Á¡) 10. Productivity¶õ ¹«¾ùÀΰ¡? (5Á¡) 11. Enzyme kinetics·ÎºÎÅÍ ¹«¾ùÀ» ¾Ë ¼ö Àִ°¡? (5Á¡) 12. Polymerase chain action (PCR)¿¡ ´ëÇÏ¿© 3´Ü°è·Î ±¸ºÐÇÏ¿© ±â¼úÇϽÿÀ. (5Á¡) 13. Agarose gel electrophoresis½Ã, »ç¿ëÇÏ´Â TAE bufferÀÇ ±¸¼º¼ººÐ ¹× °¢°¢ÀÇ ¿ªÇÒ¿¡ ´ëÇØ ¾à¼úÇϽÿÀ. (5Á¡) 14. ½ÄÇ°/õ¿¬¹°À» ÀÌ¿ëÇÑ µ¿¹°½ÇÇè ±â¹ýÀÇ ÇÑ ¿¹¸¦ ¼¼úÇϽÿÀ. (5Á¡) 15. µ¿¹°½ÇÇè À±¸®¿¡ ´ëÇؼ ÀÚ½ÅÀÇ ÀÇ°ßÀ» ¼¼úÇϽÿÀ. (5Á¡) 16. Western blottingÀÇ °³¹ß·Î ÀÎÇÏ¿© »ý¸í°úÇп¡ ¾î¶°ÇÑ º¯È°¡ ÀϾ´ÂÁö¿¡ ´ëÇؼ ¾à¼úÇÏ°í ÀÌÀÇ ¿ø¸®¿¡ ´ëÇÏ¿© ¼³¸íÇϽÿÀ. (10Á¡) |