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http://feb.knu.ac.kr/cafe/?myhomeboard.278.
¡° »ýÈ­ÇÐ 1 Áß°£°í»ç (2006³âµµ) ¡±
(1) »ýüºÐÀÚ»çÀÌÀÇ °¡¿ªÀû »óÈ£ÀÛ¿ë¿¡¼­ °ü¿©ÇÏ´Â °áÇÕ 3°¡Áö¸¦ ¼³¸íÇϽÿÀ (5Á¡).
(2) »ýü ºÐÀÚ »çÀÌ¿¡¼­ÀÇ ¹°ÀÇ Æ¯¼ºÀ» ¼³¸íÇϽÿÀ (5Á¡)
(3) »ýüÀÇ ¸ðµç »ýÈ­ÇÐ ¹ÝÀÀÀÌ ¿­¿ªÇÐ ¹ýÄ¢¿¡ ÀÇÇÏ¿© ¼³¸í °¡´ÉÇÑ°¡? (5Á¡)
(4) ¿Ö RNA ºÐÀÚº¸´Ù enzymeÀÌ biocatalyst·Î ÁøÈ­°úÁ¤¿¡¼­ ¼±º°µÇ¾ú´Ù°í ÆǴܵdzª? (5Á¡)
(5) ´ÙÀ½ÀÇ ¾Æ¹Ì³ë»ê ¼ø¼­¸¦ ÇÑ ±ÛÀڷΠǥ±âÇÏ´Â ¾ÏÈ£¹®À¸·Î ¹ø¿ªÇϽÿÀ (5Á¡).
Leu-Glu-Ala-Arg-Asn-IIe-Asn-Gly-Ser-Cys-IIe-Glu-Asn-Cys-Glu-Ile-Ser-Gly-Arg-Glu-Ala-Thr
(6) ´ÙÀ½ÀÇ ¾Æ¹Ì³ë»ê Áß ÀÌµé ¾Æ¹Ì³ë»êÀÌ °¢°¢ÀÇ polypeptide ¶Ç´Â proteinÀÇ ¼ººÐÀ¸·Î µÇ¾î ÀÖÀ» °æ¿ì¸¦ °¡Á¤ÇÏÀÚ. ±×·¯¸é, ÁöÁú2ÁßÃþ¸·(lipid bilayer)ÀÇ Áß°£ÀÇ ¼Ò¼ö¼º ¿µ¿ª¿¡ À§Ä¡ÇÒ °ÍÀ̶ó »ý°¢µÇ´Â ¾Æ¹Ì³ë»ê¿¡ ¸ðµÎ O Ç¥¸¦ ÇϽÿÀ (10Á¡).
           Phe   Arg   Met   Asp    IIe     Lys    Leu    Val     Gly     Ala  
(7) Ribonuclease¿¡ ¾î¶² ¹°ÁúÀ» ÷°¡ÇÏ¿´´õ´Ï À̵éÀÇ È¿¼Ò È°¼ºÀÌ ¼Ò½ÇµÇ¾ú´Ù°í ÇÑ´Ù. ¾î¶² ¹°ÁúÀΰ¡? (2Á¡)  ¶ÇÇÑ À̵鿡 ¾î¶² °úÁ¤À» °ÅÄ¡°Ô ÇÏ´Ï ´Ù½Ã È°¼ºÀÌ µ¹¾Æ ¿Ô´Ù°í ÇÑ´Ù. ¾î¶² °úÁ¤ÀÎÁö ¼³¸íÇϽÿÀ (3Á¡).  
(8) ´Ü¹éÁú¿¡ ÀÖ´Â °ÅÀÇ ¸ðµç °áÇÕÀº TransÇüÀÎ ÀÌÀ¯´Â? (5Á¡)
(9) alpha-helix ±¸Á¶¸¦ ¼³¸íÇ쵂 ±¸Á¶ÀÇ ¾ÈÁ¤¼ºÀ» °­Á¶ÇÏ´Â °áÇÕÀ» ¹Ýµå½Ã ¼³¸íÇϽÿÀ (10Á¡).
(10) ¥â-sheet ±¸Á¶¿¡´Â ¸î °¡Áö·Î ºÐ·ùÇÒ ¼ö ÀÖ´Ù. mixed ¥â-sheet ±¸Á¶³ª twisted ¥â-sheet ±¸Á¶°¡ ³ªÅ¸³ª´Â ÀÌÀ¯´Â? (10Á¡)
(11) Reverse turnÀº ¥â turn ¶Ç´Â hairpin turn À̶ó°íµµ ÇÑ´Ù. ¶ÇÇÑ ¥Ø loop¸¦ Çü¼ºÇϱ⵵ Çϴµ¥ ÀÌ·± ±¸Á¶ÀÇ ¿ªÇÒÀº ¹«¾ùÀÎÁö ±×¸²À¸·Î ¼³¸íÇϽÿÀ (10Á¡)
(12) MyoglobinÀÇ 3Â÷±¸Á¶¿¡¼­ ÃÎÃÎÇÏ°í º¹ÀâÇÑ ³»ºÎ±¸Á¶¿¡´Â Åë»ó hydrophobic ÇÑ ºÎºÐÀ¸·Î ±¸¼ºµÇ¾î ÀÖÀ¸³ª  polar residue¸¦ Áö´Ï´Â His Àܱ⵵ 2°³ ³ªÅ¸³­´Ù. À̵éÀÇ ¿ªÇÒÀº? (10Á¡)
(13) ´Ü¹éÁúÀÇ Á¤Á¦ÀÇ °¡Àå óÀ½ ´Ü°è·Î ¿°¼®(salting out)À» µé ¼ö ÀÖ´Ù. ´ëºÎºÐ ´Ü¹éÁúÀº ³ôÀº ¿° ³óµµ¿¡¼­ ¿ëÇصµ°¡ °¨¼ÒÇÏ¿© 0.8-2.4M ÀÇ ammonium sulfate¸¦ ÷°¡ÇÏ¸é ´Ü¹éÁúÀÌ Ä§ÀüµÈ´Ù. ±×·¯¸é ÀÌ Ä§Àü¹°¿¡¼­ ¿°À» Á¦°ÅÇÏ·Á¸é ¾î¶»°Ô ÇÏ´ÂÁö ±×¸²À¸·Î ¼³¸íÇϽÿÀ (10Á¡).
(14) ÇÑ ´Ü¹éÁúÀÇ ÀüüÀûÀÎ net charge°¡ °­ÇÑ ¿°±â¼ºÀ» ³ªÅ¸³¾ ¶§ ´Ü¹éÁú Á¤Á¦ Áß ¾î¶² ion exchanger¸¦ »ç¿ëÇÏ¿©¾ß Çϳª? (5Á¡)
(15) Gel filtrationÀÇ ¿ø¸®¸¦ ÀûÀ¸½Ã¿À (10Á¡).
(16) glycoproteinÀ» Á¤Á¦ÇÏ´Â µ¥ °¡Àå ¸¹ÀÌ »ç¿ëÇÏ´Â Á¤Á¦ÀÇ ¹æ¹ýÀ¸·Î affinity chromatography°¡ ÀÖ´Ù. ÀÌ ¹æ¹ýÀ» ¼³¸íÇϽÿÀ (10Á¡).
(17) gel electrophoresis´Â ´Ü¹éÁú, DNA, RNA¸¦ ºÐ¸®ÇÏ´Â È¿°úÀûÀÎ ¼ö´ÜÀÌ´Ù. ÀÌ ¹æ¹ýÀÇ ¿ø¸®¸¦ ¼³¸íÇϽÿÀ (10Á¡).
(18) ultracentrifugation¿¡¼­ gradient¸¦ ¸¸µå´Âµ¥ ¾î¶² ¹°ÁúÀ» »ç¿ëÇϳª? (5Á¡)
(19) Saccharomyces cerevisiae (yeast)¸¦ ¹è¾çÇϸ鼭 heat shock¸¦ Á־ ¿©±â¼­ ¹ßÇöÇÏ´Â heat shock protein (HSP)-30 (¾à 30 kD)°ú HSP-70 (¾à 70 kD)À» 2-D electrophoresis¸¦ °ÅÄ¡°í Matrix-assisted laser desorption-ionization (MALDI)-TOF (Time Of Fly)·Î ¹ßÇö À¯¹«¸¦ Á¤¼º È®ÀÎÇÏ·Á°í ÇÑ´Ù. Àü °úÁ¤À» ±×¸²À» ±×·Á ¼³¸íÇϽÿÀ (20Á¡).
(20) ¾Æ¹Ì³ë»êÀÇ ¼ø¼­ (amino acid sequence)°¡ Áß¿äÇÑ ÀÌÀ¯¸¦ 3°¡Áö ÀÌ»ó ¼³¸íÇϽÿÀ (5Á¡).
(21) monoclonal antibody Á¦ÀÛÀÇ ¿ø¸®¸¦ ¼³¸íÇϽÿÀ (10Á¡).
(22) Sandwich ELISAÀÇ ÀåÁ¡Àº ¹«¾ùÀΰ¡? (5Á¡)
(23) Western blottingÀº ´Ü¹éÁúÀÇ °ËÃâ¿¡ À¯¿ëÇÑ ¹æ¹ýÀÌ´Ù. ÀÌÀÇ ½ÇÇè¹æ¹ý¿¡ ´ëÇÏ¿© ¼³¸íÇϽÿÀ (10Á¡).
(24) fluorescence microscopy´Â ¼¼Æ÷¸¦ Çü±¤À¸·Î Ç¥ÁöÇÑ Ç×ü³ª Çü±¤´Ü¹éÁú·Î Âø»öÇÏ¿© ¿ì¸®°¡ °ü½ÉÀÖ´Â ´Ü¹éÁúÀÇ localizationÀÇ ¿¬±¸¿¡ »ç¿ëÇÑ´Ù. ÀÌ·± ¹æ¹ýÀÇ ÀåÁ¡Àº ¹«¾ùÀΰ¡? (5Á¡)
(25) ´Ü¹éÁúÀ» Á¤·®ÇÒ ¶§ 280 nm¿¡¼­ spectrophotometer¸¦ »ç¿ëÇÑ´Ù.
ÀÌ ¹æ¹ýÀÇ ¿ø¸®´Â? (5Á¡)
(26) Complete the table below (10Á¡).
--------------------------------------------------------------------------
purification      total protein   total activity    Specific activity   purification     Yield
procedure          (mg)          (U)              (U/mg)         level          (%)
--------------------------------------------------------------------------
Crude extract     20,000        4,000,000
(NH4)2SO4 ppt.    5,000        3,000,000    
DEAE-cellulose   1,500        1,000,000
gel-filtration            500          750,000
affinity                     45          675,000
--------------------------------------------------------------------------
´ÙÀ½¿¡ ÀûÀýÇÑ ¿µ¾î ´Ü¾î¸¦ ³ÖÀ¸½Ã¿À (°¢ 2Á¡).
(27) Three-dimensional protein structure  can be determined by NMR spectroscopy
and (                    ).
(28) Proteins are built from a repertoire of (              ).  (            ) are linear polymers built of monomer units called amino acids.
(29) Some polypeptide chains fold into two or more compact regions that may be connected by a flexible segment of polypeptide chain, rather like pearls on a string.  These compact globular units, called (            ), range in size from about 30 to 400 amino acid residues.  
(30) An antibody is a protein synthesized by an animal in response to the presence of a foreign substance, called an (        ), and normally functions to protect the animal from infection.
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