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“ 생화학 1 기말고사 (2006년도) ”
1. 긴 DNA stretch일 경우에 chromosome walking에 의하여 분석이 가능하다. 이를 library screening의 한부분이라 생각하고 전체적인 흐름도(flowchart)를 그림으로 설명하여 보시오. 2. PCR은 분자생물학에 필수기법이다. 이의 ①원리를 그림으로 그려 설명하고 ②농업생명공학에의 응용면에 대하여 설명하시오. 3. 식물체와 동물체에 있어서 glucose의 ①저장 형태의 차이와 ②생물학적 기능의 차이에 대하여 설명하시오. 4. 동물세포에 있어서 핵으로부터 단백질이 합성되어 당이 부가되는 과정을 설명하되, nucleus, endoplasmic reticulum, golgi body, cell membrane을 포함하여 그림으로 나타내시오. 5. Give an appropriate word(s) in the following sentences. ① [ ] are anionic polysaccharides chains made of repeating disaccharide units containing a derivative of an amino sugar. ② One of the most powerful methods developed to date for this purpose is based on hybridization. High-density arrays of nucleotides, called [ ], can be constructed either through light-directed chemical synthesis carried out with photolithographic microfabrication techniques used in the semiconductor industry. ③ [ ] recognize specific base sequences in double-helical DNA and cleave both strands of a duplex sequence. ④ [ ] is a highly effective alternative to autoradiography. A tag is attached to an oligonucleotide priming fragment-a differently colored one in each of the four chain-terminating reaction mixtures. ⑤ Carbohydrates may be linked to proteins through [ ] (N-linked) or through [ ] or [ ] (O-linked) residues. ⑥ A properly folded glycoprotein will move to the Golgi complex after the removal of glucose moieties. An unfolded or misfolded protein will receive a glucose residue, through the action of a glucosyltransferase. Such glucosylated glycoproteins bind to calnexin (or the related protein calreticulin), which serves as a [ ] to allow multiple attempts to attain correct folding. ⑦ [ ] are specific carbohydrate-binding proteins which promote interactions between cells. |
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